Journal: Molecular Medicine Reports

Article Title: Resveratrol protects late endothelial progenitor cells from TNF-α-induced inflammatory damage by upregulating Krüppel-like factor-2

doi: 10.3892/mmr.2018.8621

Figure Lengend Snippet: Effect of RES on KLF2 mRNA and protein expression in EPCs. (A) KLF2 mRNA expression was determined by reverse transcription-quantitative polymerase chain reaction. (B) KLF2 protein expression was determined by western blot analysis. For western blotting, EPCs were pretreated with RES at concentrations of 5, 10, 20 and 50 µmol/l for 12 h, and subsequently, EPCs were cultured with 10 ng/ml TNF-α for 24 h. β-actin was analyzed as a loading control. (C) Densitometric analysis for the western blot was performed by GelPro Analyser 4.0 software. EPCs treated with DMSO served as the blank group. *P<0.05 and **P<0.01, as indicated. In C, *P<0.05 vs. DMSO groups, ## P<0.01 vs. TNF-α groups. RES, resveratrol; KLF2, Krüppel-like factor-2; EPCs, endothelial progenitor cells; DMSO, dimethyl sulfoxide; TNF-α, tumor necrosis factor-α; R, resveratrol; T, tumor necrosis factor-α.

Article Snippet: Finally, a densitometric analysis for the western blot was performed by GelPro Analyser 4.0 software (Media Cybernetics, Inc., Rockville, MD, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Cell Culture, Software